生体にはあまり要らないと考えられていたホウ酸塩が、プロテイン内の鉄輸送に利用されているという論文
Communication
Borate as a Synergistic Anion for Marinobacter algicola Ferric Binding Protein, FbpA: A Role for Boron in Iron Transport in Marine Life
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† Department of Chemistry, Duke University, Durham, North Carolina, 27708-0346, United States
‡ Department of Chemistry and Biochemistry, San Diego State University, San Diego, California 92182-1030, United States
§ Department of Biology, Chatham University, Pittsburgh, Pennsylvania 15232, United States
J. Am. Chem. Soc., 2013, 135 (39), pp 14504–14507
DOI: 10.1021/ja406609s
Publication Date (Web): September 12, 2013
Copyright © 2013 American Chemical Society
Abstract
Boron in the ocean is generally considered a nonbiological element due to its relatively high concentration (0.4 mM) and depth independent concentration profile. Here we report an unexpected role for boron in the iron transport system of the marine bacteriumMarinobacter algicola. Proteome analysis under varying boron concentrations revealed that the periplasmic ferric binding protein (Mb-FbpA) was among the proteins whose expression was most affected, strongly implicating the involvement of boron in iron utilization. Here we show that boron facilitates Fe3+ sequestration by Mb-FbpA at pH 8 (oceanic pH) by acting as a synergistic anion (B(OH)41–). Fe3+ sequestration does not occur at pH 6.5 where boric acid (B(OH)3; pKa = 8.55) is the predominant species. Borate anion is also shown to bind to apo-Mb-FbpA with mM affinity at pH 8, consistent with the biological relevance implied from boron’s oceanic concentration (0.4 mM). Borate is among those synergistic anions tested which support the strongest Fe3+ binding to Mb-FbpA, where the range of anion dependent affinity constants is log K′eff = 21–22. Since the pKa of boric acid (8.55) lies near the pH of ocean water, changes in oceanic pH, as a consequence of fluctuations in atmospheric CO2, may perturb iron uptake in many marine heterotrophic bacteria due to a decrease in oceanic borate anion concentration.
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